PHYTOCHEMICAL ANALYSIS OF Alpinia officinarum AND TO TEST ITS ANTI - OXIDANT, ANTI-MICROBIAL, ANTI-INFLAMMATORY AND ANTI - ARTHRITIC ACTIVITY

Phytochemistry

Alpinia officinarum also known as sitharathai in Tamil belongs to the family Zingiberaceae. It is a traditional herb where the rhizome of the plant is mainly used to treat sore throat, cough and chest congestion. In this study, the phytochemicals analysis, the antimicrobial, anti-oxidant, anti- arthritics, anti-inflammatory were done using rhizome extract of Alpinia officinarum. The present study also proves that that it has many bioactive compounds like flavonoids, alkaloids, tannins, steroid and phenols justifies the use of rhizome extraction of the plant for the treatment of various diseases. Among all the properties that the extract possesses, the anti-oxidant activity was to be specifically high done by FRAP assay and DPPH assay. The antimicrobial activity was done against 3 gram-positive bacteria, 3 gram-negative bacteria and 2 fungi. Among which a fungi Aspergillus flavus showed highest zone of inhibition of 34 mm followed by bacteria Enterobacter faecium and Klebsiella pneumoniae with a zone of inhibition of 27 mm. The anti-arthritic activity was done by Bovine serum protein Denaturation method and Egg albumin Denaturation method and the result was 77.62% and 37.58% which was found to be considerably similar to that of the standard Diclofenac sodium i.e., 73.44% and 26.0%. The anti-inflammatory activity was done where the membrane stabilization and hemolytic activity was checked. It was found that as the concentration is increased from 500, 750 and 1000 µg/ml; the membrane stabilization is increased with decreasing hemolysis. Thus the study justifies the use of the plant as traditional medicine for treatment of various ailments.

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