PHYTOCHEMICAL SCREENING, FORMULATION AND EVALUATION OF ANTIBACTERIAL CREAM CONTAINING EPIPHYLLUM OXYPETALUM EXTRACT

B-Pharmacy

Background: Epiphyllum oxypetalum (Queen of the Night) is a night-blooming cactus known for its medicinal properties, particularly antimicrobial activity. Objective: This study aims to investigate the phytochemical constituents and antimicrobial efficacy of E. oxypetalum extract, and to formulate and evaluate an antibacterial herbal cream incorporating the extract. Methods: Phytochemical screening was performed to identify bioactive compounds such as alkaloids, flavonoids, tannins, saponins, and terpenoids. Ash value and acid-insoluble ash were determined to assess plant material quality. Advanced techniques including Thin Layer Chromatography (TLC), Paper Chromatography, and Column Chromatography were employed for phytochemical analysis, with further characterization via Gas Chromatography–Mass Spectrometry (GC-MS). An antibacterial cream was formulated using the extract and subjected to evaluations including PH, spreadability, viscosity, stability, and microbial efficacy. Antibacterial activity was assessed using the agar well diffusion method against Staphylococcus aureus and Escherichia coli. Results: GC-MS identified several antimicrobial bioactives. The formulated cream exhibited significant antibacterial activity, indicating its potential use in therapeutic skincare. Conclusion: The study supports the use of E. oxypetalum in herbal antibacterial formulations, highlighting the value of traditional medicinal plants in modern dermatological applications.

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