Investigation Of The Antidiabetic Activity Of Cnicus Benedictus L. In Rats

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Cnicus benedictus L., commonly known as Blessed Thistle, has been traditionally used in herbal medicine for its diverse pharmacological properties. This study investigates the antidiabetic activity of Cnicus benedictus L. in rat models, focusing on its potential to regulate blood glucose levels, improve insulin sensitivity, and mitigate diabetes-related oxidative stress. The bioactive compounds of the plant, including flavonoids and sesquiterpene lactones, are hypothesized to play a pivotal role in these effects. Experimental studies on diabetic rats demonstrated significant reductions in fasting blood glucose and improvements in lipid profiles, with additional evidence of antioxidant and anti-inflammatory activity. These findings highlight the therapeutic potential of Cnicus benedictus L. as a natural alternative for diabetes management. However, further research, including clinical trials, is essential to establish its efficacy, safety, and mechanisms of action in humans. The present study aimed to evaluate the efficacy of the Cnicus benedictus leaf methanolic extract (CBHE) in reducing the growth of cervical cancer cells (Hela cancer cell line). Methods: The extraction was achieved using the Soxhlet apparatus. The study utilized a human cervical cancer cell line for antiproliferative evaluation and a human fibroblast cell line for toxicity assessment on normal cells. The incubation periods were 24 and 72 hours, and the concentration of the extract varied between 0.1 and 1,000 µg/ml. Results: The study exhibits that the methanolic extract of Cnicus Benedictus leaves can lessen the growth of human cervical cancer cells. The growth inhibition of the extract was dependent on the concentration and time, with the highest inhibition rate seen at 1,000 µg/ml after 72 hours of incubation. The study also revealed that the extract had minimal impact on the growth of normal cells. Conclusion: The study shows that Cnicus Benedictus leaves methanolic extract has ability to inhibit the growth of human cervical cancer cells in vitro. The extract cytotoxic behavior was (cell cycle and cell non-cycle) specific. The research also found that the extract selectively kills cancer cells rather than normal cells, indicating its safety in their effectivity.

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