HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW

VISHAL NILKANTHRAO CHAVAN

HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW

June 2024

Vol-10, Issue-3

Paper ID: 24278

ISSN: 2395-4396

Downloads: 0

Abstract & Details

Research Area

Pharmacy

Keywords

Pressure Liquid Chromatography Chromatography Method validation Method development HighPressure Liquid Chromatography (HPLC) Method validation Method development

Abstract

High-Performance Liquid Chromatography (HPLC) is a type of column chromatography commonly used in biochemistry and analytical chemistry to separate, identify, and quantify active compounds. HPLC is the most frequently employed separation technology for detecting, separating, and quantifying drugs. Method development and validation of HPLC are essential in new drug discovery, development, and manufacturing, as well as various other human and animal studies. The purpose of this article is to review the method development, optimization, and validation processes of HPLC. To optimize the method, several chromatographic parameters were investigated, including sample pretreatment, mobile phase selection, column selection, and detector selection. The HPLC method can be used to analyze the majority of drugs in multicomponent dosage forms due to its advantages such as rapidity, specificity, accuracy, precision, and ease of automation.

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Author Information

#	Name	Institute / Affiliation
1	VISHAL NILKANTHRAO CHAVAN	Loknete shri Dadapatil pharate college of Pharmacy. Mandavgan, Pharata, Shirur, Pune.

How to Cite

Use the following formats to cite this article in your research.

APA Style

CHAVAN, VISHAL NILKANTHRAO (2024). HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW. International Journal of Advance Research and Innovative Ideas In Education, 10(3), 5569-5580.

MLA Style

CHAVAN, VISHAL NILKANTHRAO. "HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW." International Journal of Advance Research and Innovative Ideas In Education, vol. 10, no. 3, 2024, pp. 5569-5580.

IEEE Style

VISHAL NILKANTHRAO CHAVAN, "HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW," International Journal of Advance Research and Innovative Ideas In Education, vol. 10, no. 3, pp. 5569-5580, 2024.

Vancouver Style

CHAVAN VISHAL NILKANTHRAO. HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW. International Journal of Advance Research and Innovative Ideas In Education. 2024;10(3):5569-5580.

Harvard Style

CHAVAN, VISHAL NILKANTHRAO (2024) 'HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW', International Journal of Advance Research and Innovative Ideas In Education, 10(3), pp. 5569-5580.

Chicago Style

CHAVAN, VISHAL NILKANTHRAO. "HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW." International Journal of Advance Research and Innovative Ideas In Education 10, no. 3 (2024): 5569-5580.

Turabian Style

CHAVAN, VISHAL NILKANTHRAO. "HPLC METHOD DEVELOPMENT AND VALIDATION: A REVIEW." International Journal of Advance Research and Innovative Ideas In Education 10, no. 3 (2024): 5569-5580.