A Review on Isolation, Identification and Cellulolytic Potential of Cellulolytic Bacteria

Science

Generally, plant cellulose accounts for 30% to 50% of the dry weight of plants. Cellulose is the main component of plant cell walls. Plant species, age, tissue type, and environmental factors like temperature, light, and water availability can affect a plant's cellulose content. The major component of plant cells is cellulose, and it is quite difficult to degrade. A wide range of cellulose-degrading microorganisms have been identified, mainly fungi and bacteria that play an important role in cellulose-degradation. The cellulase enzyme produced by different microbes differs in structure and action method. Bacteria that degrade cellulose were isolated from samples acquired from diverse sites. Cellulose-degrading bacteria were isolated from diverse samples using various media and cellulose and carboxymethyl cellulose as the only carbon sources for bacterial growth. Congo red and gram iodine were used to examine isolated bacteria, followed by the detection of hydrolytic zones around cellulose-degrading bacteria. These zones were used for determining the hydrolytic potential of various bacterial colonies, and morphological and biochemical tests were carried out to determine the bacterial colony's identification. According to morphological and biochemical characterization, the isolates were identified as Bacillus subtilis, Bacillus thuringeinsis, Brevibacillus brevis, Brevibacillus parabrevis, Pseudomonas sp, Staphylococcus aureus, Staphylococcus epidermis, Salmonella sp, Klebsiella sp, Aeromonas sp, Pasteurella sp, Brucella sp, Micrococcus sp, Xanthomonas sp, Achromobacter sp,.

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