REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE
Abstract & Details
Research Area
Plant Biotechnology
Keywords
in vitro germination
Leptolaena multiflora
mature zygotic embryo
regeneration
Abstract
Leptolaena multiflora (L.) is endemic specie from Madagascar, renowned for its medicinal properties. It is listed as endangered on the IUCN red list. The seed surrounded by a hard shell is one of the limiting factors of its germination. This study aims to regenerate this specie by in vitro germination techniques and culture of mature zygotic embryo. The evaluation of the effects of types of seed scarification on germination shows that soaking seeds for 48 h improves the rate of seed germination. Besides, the increasing concentration of sucrose from 10 g/l to 40 g/l has a positive effect on the development of the embryo, and promotes the development of the aerial part and the root system of vitroplants. However, the high concentration above 80 g/l reduced the growth and in vitro development of the embryo. Beyond this dose, regeneration and root growth are weak and tend to stop.
License
This work is licensed under a Creative
Commons
Attribution-ShareAlike 4.0 International License.
Author Information
| # | Name | Institute / Affiliation |
|---|---|---|
| 1 | DINAHARILALA Mamisitraka Théo. Gloriat | Plant Physiology Laboratory, unit : Plant Regeneration and micropropagation Laboratory, Faculty of Sciences, University of Antananarivo Madagascar |
| 2 | RAVONIARISON. N | Plant Physiology Laboratory, unit : Plant Regeneration and micropropagation Laboratory, Faculty of Sciences, University of Antananarivo Madagascar |
How to Cite
Use the following formats to cite this article in your research.
APA Style
Gloriat, DINAHARILALA Mamisitraka Théo. & N, RAVONIARISON. (2023). REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE. International Journal of Advance Research and Innovative Ideas In Education, 9(4), 2143-2151.
MLA Style
Gloriat, DINAHARILALA Mamisitraka Théo., and RAVONIARISON. N. "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE." International Journal of Advance Research and Innovative Ideas In Education, vol. 9, no. 4, 2023, pp. 2143-2151.
IEEE Style
DINAHARILALA Mamisitraka Théo. Gloriat and RAVONIARISON. N, "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE," International Journal of Advance Research and Innovative Ideas In Education, vol. 9, no. 4, pp. 2143-2151, 2023.
Vancouver Style
Gloriat DINAHARILALA Mamisitraka Théo., N RAVONIARISON.. REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE. International Journal of Advance Research and Innovative Ideas In Education. 2023;9(4):2143-2151.
Harvard Style
Gloriat, DINAHARILALA Mamisitraka Théo. & N, RAVONIARISON. (2023) 'REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE', International Journal of Advance Research and Innovative Ideas In Education, 9(4), pp. 2143-2151.
Chicago Style
Gloriat, DINAHARILALA Mamisitraka Théo. and RAVONIARISON. N. "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE." International Journal of Advance Research and Innovative Ideas In Education 9, no. 4 (2023): 2143-2151.
Turabian Style
Gloriat, DINAHARILALA Mamisitraka Théo. and RAVONIARISON. N. "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE." International Journal of Advance Research and Innovative Ideas In Education 9, no. 4 (2023): 2143-2151.
Related Research
Valorization of cocoa pods (Theobroma cacao) for feeding greenhouse ducks in the Antalaha District
PDF Unavailable
Production of green energy for its economic and environmental benefits in order to guarantee human health
PDF Unavailable