Vitamin D Deficiency Induced Muscle Wasting

biotechnology

When it comes to animals and people alike, muscle loss is caused by a shortage in vitamin D. Male Sprague Dawley rats were used to establish a vitamin D-deficient rat model. Muscle atrophy caused by vitamin D insufficiency is linked to the ubiquitin proteasome and other proteolytic processes. A group of D-deficient rats was given high calcium alone to study the impact of hypocalcemia that comes along with D deficiency. HPLC was used to measure 3-methylhistidine in urine and the myosin ATPase staining procedure was used for muscle histology. Semiquantitative RTPCR was used to assess muscle gene expression. Three-methyl histidine concentrations in urine and total muscle protein breakdown were higher in the deficient group than the control group. Genes for muscle atrophy markers and proteasomal subunits were upregulated in D-deficient muscle, but genes for myogenic growth were downregulated. According to the findings, vitamin D deficiency-induced muscle protein breakdown is mostly caused via the ubiquitin proteasome pathway, and calcium supplementation alone, in the absence of vitamin D, partly corrects the abnormalities.

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