EVALUATION OF ANTIOXIDANT ACTION OF MIXED EXTRACTS OF PLUMERIA PUDICA AND ARAUCARIA COLUMNARIS

Pharmacology

The leaves of Plumeria pudica grow whirling around the stem and have a sessile base. The branchlets of Araucaria columnaris grow in whorls and are having small green, spirally arranged leaves. The extraction yield in methanol was found to be 39.6 % for Plumeria pudica and 43.7 % for Aruacaria columnaris the findings of preliminary phytochemical analysis suggest the presence of alkaloids, saponin glycosides, phenolics, terpenoids, and flavonoids in the leaf of the Plumeria pudica while alkaloids were not found to be present in Araucaria columnaris. The total phenolic content of the methanolic extracts of P. pudica and A. columnaris were 38.31±1.7 and 54.27±2.1GAE mg/g, respectively. The extracts were individually and in combination (1:1, 1:2 & 2:1) subjected to in vitro determination of antioxidant potential in terms of hydrogen donating or radical scavenging ability using the stable free radical DPPH, reducing power using potassium ferrocyanide, hydroxy radical scavenging using Iron-EDTA and ammonium molybdate method. The IC50 value of the DPPH scavenging potential for P. pudica and A. columnaris was found to be 209.3 and 194.4 µg/mL respectively. All the ratios of the extracts exhibited increase in DPPH scavenging potential, the 1:2 ratio was found to be the most synergistically potent with and IC50 value of 56.39 µg/mL. The change in absorbance was observed with increasing the concentration of the extracts ranging from 0.031-0.174 for P. pudica and 0.028-0.135 for A.columnaris in concentration range from50-250µg/mL. On combining the extracts, the extract combination of the ratio 2:1 had reduction potential at dose 150µg/mL better that ascorbic acid. The IC50 value of the percent HRSA for the P. pudica and A. columnaris were found to be 188.17 and 231.95 µg/mL respectively. The IC50 value was found to be 154.86, 137.36 and 125µg/mL for 1:1, 1:2 and 2: 1 ratio of extracts respectively. The IC50 value of the percent reduction of phosphomolybdenum for the P. pudica and A. columnaris were found to be 234.37 and 194.44 µg/mL respectively. The IC50 value of the percent PRP for the combined extracts was found to be 227.27, 170.73, and 206.42µg/mL respectively for 1:1, 1:2 and 2:1 ratios of the extracts. It was found that the 1:2 ratio the extracts were best able to inhibit the phosphomolybdenum complex.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.