EVALUATION OF ANTIOXIDANT ACTION OF MELALEUCA BRACTEATA F. MUELL. LEAF EXTRACT

Pharmacy

The leaves of the plant grow alternate, are sessile and spirally arranged. The leaves are narrow-ovate or elliptically lanceolate, 1-2 cm long and glabrous with 5-11 veins. The apex of the leaf is acute to acuminate. The extraction yield of the leaf using different solvents is presented in Figure 5.3. The extraction abilities of different solvents were found in the order: ethanol (46.3%)>water (16.7)>chloroform (10.5)>n-hexane (6.3). The findings of the phytochemical analysis suggest the presence of saponin glycosides, phenolics, terpenoids, protein, and flavonoids in the leaves. The total phenolic content of n-hexane, chloroform, ethanolic and aqueous extract of M. bracteata were 7.39±0.91, 48.31±0.26, 74.27±0.59 and 51.83±0.44 GAE mg/g, GAE mg/g, respectively. All the extract of the plant was subjected to in vitro determination of antioxidant potential. The IC50 value of the DPPH scavenging potential for n-hexane extract was found to be more than 250µg/mL whereas for chloroform, ethanol and aqueous extracts it was found to be 152.17, 89.28 and 166.46 µg/mL respectively. The reducing potential was found to be dose dependent and the followed the order ethanolic>aqueous>chloroform>n-hexane. The IC50 value of the percent HRSA for the n-hexane, chloroform, ethanol and aqueous extracts was found to be 250.0, 226.13, 164.31 and 134.4µg/mL respectively. The IC50 value of the percent PRP for the chloroform, methanol and aqueous extracts was found to be 243.24, 169.90, and 210.28µg/mL respectively while the IC50 for n-hexane extract was more than 250µg/mL.

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