EVALUATION OF ANTIDIABETIC ACTION OF ARAUCARIA COLUMNARIS LEAF EXTRACTS

Pharmacy

The leaf bearing branchlets are narrow at the petiole, broaden towards the middle and narrow down towards the apex. The ability of different solvents to extract phytochemicals from leaves followed the order: methanol>water>ethyl acetate>benzene. The preliminary qualitative phytochemical analysis suggests the presence of saponin glycosides, cardiac glycosides, phenolics, terpenoids, sterols, and flavonoids in the leaves. The total phenolic content of benzene, ethyl acetate, methanolic and aqueous extract of Araucaria columnaris were 5.18±0.91, 8.97±0.17, 63.22±0.48 and 38.24±0.63 GAE mg/g, respectively. All the extracts were subjected to in vitro determination of antioxidant potential using DPPH scavenging method. The IC50 value of the DPPH scavenging potential for benzene and ethyl acetate was found to be more than 250µg/mL whereas for methanol and aqueous extracts was found to be 136.6 and 200.2 µg/mL respectively. Alloxan induced diabetes model was used for determining the antidiabetic potential of the methanolic and aqueous extracts. The aqueous extract was able to lower the blood glucose more in comparison to the methanolic extract comparable to the standard drug glibenclamide.

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