The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film

APPLIED CHEMISTRY

A Potentiometric biosensor based on bovine serum albumin (BSA) embedded surface modified PANI/2AP has been developed for the detection of urea in water sample. The enzyme, urease (Urs), was covalently linked to free amino groups present over the BSA embedded modified surface of the conducting PANI/2AP composite film electrochemically deposited onto an indium–tin-oxide (ITO) film. The biosensor has been characterized by UV–visible, infrared spectroscopy and SEM. Potentiometric response of the enzyme electrode (Urs/BSA- PANI/2AP /ITO) were measured as a function of urea concentration in phosphate buffer. It has been found that the electrode responds to low urea concentration with wider range of detection. The electrode showed a linear response range of current 0-30µA for 1-100mM solution of urea in PBS at pH 7.0. The response time is about 50-70 s reaching to a 90% steady-state potential value. These results indicate an efficient covalent linkage of enzyme to free amino groups of the BSA molecules over the surface of PANI/2AP composite film, which leads to high enzyme loading, an increased lifetime stability of the electrode and an improved wide range of detection of low urea concentration in aqueous medium.

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