CHARACTERIZATION OF FATTY ACIDS AND ANTIOXIDANT POTENTIAL ANALYSIS IN BAHUNIA RACIMOSA AND CASSIA FISTULA SEEDS OIL

Chemistry

Physical and chemical characteristics, fatty acid composition, antioxidant potential and total phenolic content of oil extracted from Bahunia racimosa and Csasia fistula seeds are presented in this work. The results indicate that B. racimosa and C. fistula seeds constituted 27% and 44% oil respectively. The refractive index, saponification value and iodine value of B. racimosa and C. fistula seed oils were found to be 1.425 and 1.413, 125.6 and 105, 73 and 69 respectively. Prior to GC and GC-MS analysis, the extracted oil underwent methyl esterification. The major fatty acids reported here, in B. racimosa and C. fistula seeds oil, are Myristic acid (1.3% and 0.8%), Palmitic acid (17.5% and 19.3%), Stearic acid (11.2% and 7.3%), Oleic acid (11.8% and 18.1%), Linoleic acid (55.3% and 52.2%) and Linolenic acid (1.4% and 1.1%). The antioxidant potential were analyzed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and Ferric reducing ability of plasma (FRAP) methods. The results confirmed that both the species have significant antioxidant properties. The total phenolic content in B. racimosa and C. fistula were 22.46±1.8 mg GAE/g oil and 19.82±1.2 mg GAE/g respectively. The oil from B. racimosa and C. fistula seeds could find new uses as a result of this study.

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