Expression of the srlI gene to improve salt tolerance in Escherichia coli

agriculture

In trying to engineer E. coli to enhance salt tolerance, this study shows constitutive over-expression of the srlI (glucitol-6-phosphate dehydrogenase) gene studying GMO E. coli. These genes used by studied salinity stress at five concentrations of NaCl. The srlI gene was isolated from E. coli top10 and inserted it into E. coli DH5 alpha with pET32a+ vector was used. The confirmation of transgene integration and expression were made via the optical density (OD600) that is at 250 mmol/L NaCl concentration the srlI increase significantly (0.4227) compared with control (0.3564). The bacterial colonies with the recombined plasmid in the presence of IPTG and X-gal are colored white. The genotypic diversity of isolates was done using PCR that shows the similar patterns as the pattern of whole-cell protein for SDS-PAGE. Therefore, we need to attempt to explore the physiological mechanisms of E. coli srlI gene to salt tolerance in newly-developed transgenic bacteria lines for comparison of their physiological performance in a salt-stress environment.

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