Production of Cellulase with Potential Use in Sugarcane Bagasse Saccharification by Fungi

Microbiology

The research focused on cellulase synthesis with possible application in sugarcane bagasse fungal saccharification. Under this regard, the goal is to examine the operating parameters (temperature and humidity) in which cellulases of the Penicillium sp., Rhizomucor sp. fungus are produced. INCQS 40331 (CFAM 422) and Trichoderma koningii, which use as natural sugarcane bagasse (NSB) and pretreated by acid-alkaline solution (AAB) and hydrogen peroxide (HPB), respectively. The research was conducted using three-tier factory design (32) with central points. The findings indicate that the Trichoderma koningii (8.2 IU/g substratum), followed by Penicillum sp (1.7 IU/g substratum), was the most appropriate cellulase fungus. In addition, the best results have been achieved with NSB for all fungi. Statistical analysis revealed that the temperature has a larger impact on cellulase production by the fungus assessed.

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