Bacterial Contamination of Women’s Handbags and Their Contents in Ajilat City, Libya

Biology

This study aimed to investigate the types and prevalence of bacterial contamination in women’s handbags and their commonly used contents in Ajilat City, Libya. A total of 100 samples were randomly collected from handbags, mobile phones, makeup brushes and sponges, bank cards, paper currency, and coins. Ethical approval and informed consent were obtained prior to sample collection. Samples were collected using a Z-pattern swabbing method and preserved in blood culture media for transport to the microbiology laboratory. The cotton swabs were cultured on selective and differential media, including blood agar and MacConkey agar, and incubated at 37°C for 48 hours. Bacterial colonies were examined based on morphological characteristics and microscopic analysis to identify the species present. The results revealed that 98% of handbag samples contained bacterial colonies, with Escherichia coli and Staphylococcus spp. being the most frequently identified. Mobile phones were predominantly contaminated with Staphylococcus spp., while makeup brushes and sponges were contaminated with both Streptococcus spp. and Staphylococcus spp. Escherichia coli was also detected on 50 bank card samples and in all paper currency and coin samples. These findings indicate that women’s handbags and their contents act as potential fomites, facilitating the transmission of pathogenic and opportunistic bacteria in community settings. The study highlights the importance of personal hygiene, regular cleaning of handbags and personal items, and public health awareness to reduce bacterial spread and prevent infection.

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