Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment

Microbiology

Bacterial L-asparaginase is amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukaemia. In the present study, alkaline isolates were screened for potential producers of L-asparaginase using L-asparagine major source in M9 medium and phenol red as indicator. Further the isolates were been studied morphologically, biochemically to identify Bacillus sp, a novel strain. It produced largest zone of hydrolysis due to the bacteria utilised aspargine as nitrogen source. Bacillus sp. was explored for the production of extra-cellular L-asparaginase enzyme. The enzyme production was carried out by submerged fermentation. The studied strains shows prominant enzyme activity as mesophillic Bacillus Sp. at pH 7.6 and temperature 700C with findings that metal ion Mg2+ as enhancer and EDTA as a inhibiter

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