ISOLATION, IDENTIFICATION AND SCREENIG OF LIPASE ACTIVITY OF BACTERIAL ISOLATE

Biotechnology

The dominant bacterial isolate was isolated from soil near active mud volcanoes ( Minbu ).Isolated strain was identified by microscopy, colony morphology a, biochemical characters and 16 s RNA sequences aligning sequences from NCBI database . According to these analysis, the isolated strain was Bacillus oceanisediminis and it has lipase enzyme on 1 % of the four oil; almond oil, groundnut oil, olive oil and sesame oil. Methyl red reagent was used for difference of clear zone. Although lipase producing activity on the four oil based media was not very different of lipase producing activity, olive oil and groundnut oil added culture media were more clear zone around bacterial streak. Some enzyme activity was tested and protease activity on litmus milk media was also observed. Bacillus oceanisediminis strain gave pink color of positive result. Growth condition of pH, temperature, and NaCl usage were broad range. In this study, dominant strains of volcanic environment was characterized and the potential activity of isolates were observed and can be used to industrial purposes, food industries and many other applications

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