Diagnosis of cervical cancer using Confocal Raman Spectroscopy

electronics and communication engineering

According to W.H.O., cervical cancer is the leading cause of fatality in women in India, though it can be effectively treated if diagnosed at an early stage. Pap smear test carried out regularly can aid in early diagnosis but the accuracy of this test is around 70%. Hence to this date, histopathology study is regarded as the golden standard but it is a cumbersome process and is left at the expertise of the pathologist. Accurate, rapid and non-invasive detection and diagnosis of malignant disease in tissues is an important goal of biomedical research and is the need of the hour. Optical methods such as diffuse reflectance, fluorescence spectroscopy and Raman spectroscopy are actively explored to this end. Raman spectroscopy, a method based on Raman scattering, is a powerful technique that can be applied to many tissue sites. Raman spectroscopy is a molecular-specific technique that probes the vibrational or rotational transitions in chemical bonds and provides detailed information about the biochemical composition of a sample. The use of Raman spectroscopy in the detection and classification of malignancy within the human uterine cervix would be evaluated in this work. The excitation wavelength being 785nm from a diode laser source is selected for this study. The wave number region to be analysed would be from 600-1800cm-1 which is the fingerprint region for bio molecules. The spectra would be corrected for noise and then analysed by statistical significances.

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