MITIGATING EFFECT OF L-ASCORBIC ACID ON DNA CONTENT ALTERATIONS CAUSED BY PROFENOFOS IN LAMELLIDENS MARGINALIS (LAMARCK)

Biology

The present study investigates the genotoxic effects of chronic exposure to the organophosphate pesticide profenofos and the potential protective role of L-ascorbic acid (vitamin C) in the freshwater bivalve Lamellidens marginalis. Bivalves were divided into control and experimental groups, with treatments including profenofos exposure alone and in combination with L-ascorbic acid (50 mg/L), followed by a recovery phase in normal and L-ascorbic acid enriched water. DNA content was quantified in various tissues (gills, gonads, digestive gland, mantle, foot, and whole soft body mass) using the diphenylamine method. Results showed a significant, time-dependent reduction in DNA content across all tissues following profenofos exposure. However, co-exposure to L-ascorbic acid significantly mitigated DNA damage, and recovery in vitamin C-treated water led to a faster and more complete restoration of DNA levels compared to natural recovery.

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