ANTIMICROBIAL ACTIVITY OF GALANGAL EXTRACT TO AGAINST Aeromonas hydrophila WHICH ATTACKED SANGKURIANG CATFISHANTIMICROBIAL ACTIVITY OF GALANGAL EXTRACT TO AGAINST Aeromonas hydrophila WHICH ATTACKED SANGKURIANG CATFISH

fisheries science

Motile Aeromonas Septicemia (MAS) is a freshwater fish disease which is often the cause of aquaculture failure. Treatment of MAS is usually done by giving antibiotics, but long-term use of antibiotics can cause residues in fish. Galangal is a natural ingredient that can be utilized because it contains active ingredients such as flavonoids, essential oils, tannins and saponins which are antibacterial. The method used is experimental with Completely randomized design (CRD) used six treatments and three repetitions for the in vitro test while for the in vivo test used six treatments and two repetitions. The in vitro test treatment was carried out at a concentration of 250 mg/L (A), 500 mg/L (B), 750 mg/L (C), 1000 mg/L (D) and 1250 mg/L (E) while the in vivo test was carried out at a concentration of 0 mg/L (A), 250 mg/L (B), 500 mg/L (C), 750 mg/L (D), 1000 mg/L (E) and 1250 mg/L (F). The bacteria used was Aeromonas hydrophila with a density of 108 CFU/mL. The sangkuriang catfish used for in vivo tests was 5-7 cm in size. Parameters observed were the diameter of the inhibition zone and fish mortality (LC50 24 hours). Inhibition zone data were analyzed using the F test and 24-hour LC50 data were analyzed descriptively. The results showed that galangal extract at a concentration of 1250 mg/L could inhibit the growth of Aeromonas hydrophila in the strong category with an average inhibition zone diameter of 11.11 mm and a 24-hour LC50 value obtained at a concentration of 760.29 mg/L The conclusion of this study is that galangal extract can be used to treat catfish infected with Aeromonas hydrophila with concentrations below 760.29 mg/L.

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