Preparation, characterization and antimicrobial potential of essential oil based nano emulsion formulated with Saponin extract

Bio Physics

Several biochemical constituent present in castor and olive oil makes it an efficient antimicrobial agent against human pathogens. The current investigation aims at assessing the antimicrobial potential of olive and castor oil nanoemulsion formulated using aqueous saponin extract. Extraction protocol for saponin from Sapindus mukorossi was standardized. Saponin was characterized through FTIR. Aqueous extraction of S.mukorossi (0.4%) was used as biosurfactant for nanoemulsification of Olive and Castor oil through ultrasonication technique. The formulated nanoemulsion were investigated for their antimicrobial activity against four human pathogens namely, S.aureus, A.buamanni, B.subtilis, E.faecalis. Inhibitory concentration of the prepared nanoemulsions were determined and minimum inhibitory concentration value for Castor Oil nanoemulsion was found to be 16µl/ml and that for Olive Oil nanoemulsion was 128µl/ml. Sapindus mukorossi can thus emerge as potential source of biosurfactant for formulating emulsion based preparations in food industry.

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