METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF REMOGLIFLOZIN ETABONATE AND METFORMIN HYDROCHLORIDE IN TABLET DOSAGE FORM BY RP-HPLC

Pharmcy

A simple, rapid and accurate RP-HPLC method was developed for simultaneous estimation of Remogliflozin etabonate and Metformin hydrochloride in tablet dosage form. A Sheisedo C18 (4.6mm X 250mm, 5µm) column was used with a flow rate of 1 ml/min and detection wavelength of 254nm. The Mobile phase used is Phosphate buffer 3.0 pH– Acetonitrile (30:70 %v/v). The retention time of Metformin hydrochloride and Remogliflozin etabonate was found to be 3.727 min and 5.007 min respectively. The linearity was observed in the concentration range of 12.5-75 μg/ml and 2.5-15 μg/ml for Metformin hydrochloride and Remogliflozin etabonate respectively. The proposed method was simple, rapid, accurate, precise and useful for the routine analysis.

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