INVESTIGATING THE RELEASE OF ETHANOL EXTRACT OF JENGKOL (ARCHIDENDRON PAUCIFLORUM) FRUIT PEEL ENCAPSULATED IN PLGA NANOPARTICLES IN SIMULATED INTESTINAL FLUID

Zoological sciences

The oral administration of herbal medicine is easier and yields excellent results; however, the release of the medicine can be hindered due to degradation processes in the digestive tract. To maintain the stability of the medicine, PLGA nanoparticle encapsulation is employed. Ethanol extract of Jengkol fruit peel (EEOJFP) is known to have potential for development as an antidiabetic agent. This study aims to determine the influence of PLGA nanoparticle encapsulation on the release concentration of EEOJFP in the digestive tract. Release testing of EEJFP from PLGA nanoparticle encapsulation was conducted in vitro in simulated intestinal fluid (Simulated Intestinal Fluid pH 6.8) over 72 hours. The EEOJFP concentration was measured using HPLC with a mobile phase of methanol and water (8:2) and a C18 column (250 mm x 4.6 mm, 5 μm pore size). The research results indicate that the concentrations of EEOJFP released were 0.03 ppm at 1 hour, 0.128 ppm at 2 hours, 0.201 ppm at 4 hours, 0.343 ppm at 8 hours, 0.374 ppm at 16 hours, 0.486 ppm at 24 hours, 0.528 ppm at 48 hours, and 1.118 ppm at 72 hours. The concentration of EEOJFP released from PLGA nanoparticle encapsulation in simulated intestinal fluid was notably low. In conclusion, PLGA nanoparticles can protect EEOJFP from degradation processes in the digestive tract.

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