IN VITRO AND EX-VIVO ANTI-OXIDANT EFFECT OF CORCHORUS OLITORIUS (JUTE) SEEDS

Life Siences

The need for more studies on antioxidant effects of Corchorus olitorius (Jute) commonly used in traditional western part of Nigeria, has been on the increase. Thus, the antioxidant effects of Jute seeds have been explained in this study. The ethanol and hexane extracts of Corchorus olitorius seeds were prepared and phytochemical constituents examined. Their ability to prevent oxidation was also examined in vitro by reacting with DPPH after observing the decolonization of the reaction. Ex vivo, the levels of MDA and the ability of the extracts to increase Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) activity was also examined. Qualitative phytochemical analysis showed that ethanol extracts had phenols, tannins, alkaloids, flavonoids, saponins and terpenoids, while hexane extracts showed alkaloids. flavonoids and terpenoids only. The results of in-vitro antioxidant assay result showed a dose dependent increase in DPPH free radical scavenging activity in both ethanol and hexane extracts. In the ex-vivo studies, Ethanol extract of MDA levels increased with increasing concentration while hexane extracts decreased with increasing concentration. Corchorus olitorius significantly increased the inhibitory percentage (%) of pyrogallol autoxidation SOD highest at ethanol extract 100mg and hexane extract 200mg, while the activity of GPx was increased significantly at hexane extracts 200mg.

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