Determination Of Changes In Oral Flora After Fixed And Removable Appliances Using Saliva Test - A Research

dentistry

Introduction : The presence of fixed appliances in the oral cavity of orthodontic patients could alter the nature of dental plaque.The structure, metabolism and composition of dental plaque would change, leading to an increase in microbial population. Moreover fixed appliances might even hamper effective oral hygiene and cause high cariogenic challenge.Furthermore, based on the difficulty of maintaining oral hygiene, the subgingival microbiota may also be influenced by orthodontic appliances,since orthodontic accessories would favour bacterial plaque retention. These variables would lead to pathogenic bacteria colonisation, which are responsible for gingival inflammation, periodontal support destruction and changes in enamel surface.hence this research is done to assess the causes for changes in oral flora due to fixed and removable appliances. Materials And Methods : Thirty patients attended to the department of orthodontics at saveetha dental college, their age ranged from 15-30 years and they were in good general health. Those patients were divided into 3 groups. The subjects were asked to spit and the samples were collected from each group using sterile urico containers ,the patient is atleast asked to spit two times, and then it’s streaked on different types of media such as Blood agar, Chocolate agar, MacConkey agar and Sabouraud media then incubated aerobically for 24 hours at 37 C in order to be examined under light microscope and biochemical tests. Finally the colony count of the organisms were noted. Conclusion:Based on the findings of the study, it is concluded that normal patients without any orthodontic treatment ,showed 40% lower colony count than that of the patients with removable appliance and 60% lower than that of the patients with fixed appliance . These Results clearly showed that an orthodontic appliance increases colonisation of bacteria in oral cavity during orthodontic treatment.

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