JEJUNUM HISTOLOGICAL PROFILE OF WISTAR MALE RATS AFTER FEEDING GADUNG (DIOSCOREA HISPIDA) TUBER BLOCK BAIT

Zoological sciences

Rodent management practices routinely involve the use of chemical products, mainly anticoagulant rodenticides (ARs). Chemical rodenticide certainly has an efffect to the enviromental such as living residues. Gadung tuber contain secondary metabolites dioscorine and cyanide acid which cause damage to the structure and function of visceral organs. The aim of this study was to determine the effect of gadung tuber block bait on the histological profile of the jejunum of Wistar male rats. The method used is an experimental method with a Completely Randomized Design (CDR). The test animals consisted of 24 male rats with an average weight of ±180 g grouped into 4 treatment groups namely KN (block bait without gadung tuber), P1 (30% gadung tuber block bait), P2 (50% gadung tuber block bait), and P3 (70% gadung tuber block bait). The treatment was given for 4 days and on the 5th day, the rats were sacrificed by neck dislocation. Furthermore, jejunum were isolated and histological preparations were made. Parameters observed were the number of goblet cells and the severity of epithelial necrosis on histological incisions. Data on the number of goblet cells were analyzed using ANOVA and Duncan, while data on the severity of epithelial necrosis were analyzed using the Kruskal Wallis test and the Mann Whitney test. The results showed that gadung tuber significantly decreased the number of goblet cells and increased the severity of jejunal epithelial necrosis. It can be concluded that Gadung tuber has the potential to be developed as a natural rodenticide with an effective concentration of 50%.

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