Strategic Purification and characterization of the antibiotic metabolite from Staphylococcus epidermedis GB-51

Marine Biology

Bacteria isolated from marine sediment has been evaluated for its bioactivity which showed broad spectrum antimicrobial activity against pathogenic microorganisms. Bioactive compound producing microbes has been identified by agar diffusion method. Secondary metabolite production of the microbe, the bioactive compound has been separated through chromatographic techniques. Column chromatography has been used for screening and purification of bioactive compound, which was characterized to be by various spectroscopic techniques includes NMR and LCMS and it was found to be apparently bioactive compound 2-[2-Amino-5-(formyl-hydroxy-amino)-pentanoylamino]-5-({2-[2-amino-5-(formyl-hydroxy amino)-pentanoylamino]-3-hydroxy-butyryl}-hydroxy-amino) pentanoic acid. Minimum Inhibitory Concentration (MIC) of the active compound has been evaluated, which was found to be comparable with the standard antibiotics. Viable microbial cell study of the active compound showed it to be bactericidal in nature. Further, the compound tested for its biosafety. Cytotoxicity studies of the compound on cancer cell lines is planned to study after the pure antibiotic compound isolation. The current study demonstrated that the bioactive compound isolated from Staphylococcus sp. seems to be a stable and potent antimicrobial.

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